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NCL Antibody - N-terminal region (ARP40583_P050)
| Datasheets/Manuals | Printable datasheet for anti-NCL (ARP40583_P050) antibody |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Yeast, Zebrafish |
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | IHC, WB |
| Additional Information | IHC Information: Paraffin embedded skin tissue, tested with an antibody dilution of 5 ug/ml. |
| Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human NCL |
| Purification | Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 87%; Dog: 93%; Horse: 93%; Human: 100%; Mouse: 91%; Pig: 86%; Rabbit: 77%; Rat: 93%; Yeast: 83%; Zebrafish: 85% |
| Peptide Sequence | Synthetic peptide located within the following region: GKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDED |
| Concentration | 0.5 mg/ml |
| Blocking Peptide | For anti-NCL (ARP40583_P050) antibody is Catalog # AAP40583 (Previous Catalog # AAPP22759) |
| Sample Type Confirmation | NCL is supported by BioGPS gene expression data to be expressed in Jurkat |
| Reference | Huang,Y., (2006) Blood 107 (9), 3564-3571 |
| Gene Symbol | NCL |
|---|---|
| Gene Full Name | Nucleolin |
| Alias Symbols | C23, Nsr1 |
| NCBI Gene Id | 4691 |
| Protein Name | Nucleolin |
| Description of Target | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. |
| Uniprot ID | P19338 |
| Protein Accession # | NP_005372 |
| Nucleotide Accession # | NM_005381 |
| Protein Size (# AA) | 710 |
| Molecular Weight | 78kDa |
| Protein Interactions | HUWE1; PIK3R1; PA2G4; NCL; HNRNPD; AURKA; MDM2; NFE4; CDKN1A; ADAP1; STAU1; ADAP2; UBC; NUP62; ZBTB1; ARFGEF1; RPA3; RPA2; RPA1; ERG; RNF2; SUZ12; EED; EZH2; BMI1; NPM3; EIF3G; XRCC5; PLOD2; NCBP1; MCM2; LMNA; PRMT1; HNRNPK; KCTD12; DNM1; ACLY; SRPK3; TAR |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "NCL Antibody - N-terminal region (ARP40583_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Yeast, Zebrafish".
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How long will it take to receive "NCL Antibody - N-terminal region (ARP40583_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "NCL Antibody - N-terminal region (ARP40583_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "NCL Antibody - N-terminal region (ARP40583_P050)"?
This target may also be called "C23, Nsr1" in publications.
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What is the shipping cost for "NCL Antibody - N-terminal region (ARP40583_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "NCL Antibody - N-terminal region (ARP40583_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "NCL Antibody - N-terminal region (ARP40583_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "78kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "NCL Antibody - N-terminal region (ARP40583_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NCL"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NCL"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NCL"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NCL"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NCL"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NCL"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
