NCKAP1 Antibody - middle region (OAAB03316)

Catalog No: OAAB03316

Size:400ul

NCKAP1 Antibody (Center) western blot analysis in A2058 cell lysates (35ug/lane).This demonstrates the NCKAP1 antibody detected the NCKAP1 protein (arrow).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB
  Cellular Location: Cell membrane; Single-pass membrane protein; Cytoplasmic side Cell projection, lamellipodium membrane; Single-pass membrane protein; Cytoplasmic side Note=At the interface between the lamellipodial actin meshwork and the membrane.
  Tissue Location: Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This NCKAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 631-659 amino acids from the Central region of human NCKAP1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Zebrafish, Mouse, Rat, Xenopus
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: NCKAP1
• Alias Symbols: HEM2, NAP1, NAP125, p125Nap1
• NCBI Gene Id: 10787
• Protein Name: Nck-associated protein 1
• Description of Target: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.
• Uniprot ID: Q9Y2A7
• Protein Accession #: NP_038464.1
• Molecular Weight: 129 kDa