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Catalog No: OKEH08702
Size:96T
Price: $725.00
SKU
OKEH08702
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology NCF2 ELISA Kit (Human) (OKEH08702) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NCF2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NCF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NCF2 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=4.9% (n = 20)
Mean Inter-assay CV%: <=6.7% (n = 20)
ELISA Kit Component
ComponentAmount
NCF2 Microplate96 Wells (12 x 8 Well strips)
NCF2 Lyophilized Standard2
100X Biotinylated NCF2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
::Subcellular Location: Cytoplasm
::Unigene: Hs.587558
SMR: P19878
String: 9606.ENSP00000356505
::KEGG: hsa:4688
MIM: 233710
Pfam: PF00564
Reconstitution and StorageStore as indicated in product manual.
Sensitivity39.3pg/mL
SpecificityNatural and recombinant Human Neutrophil cytosol factor 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNCF2
Gene Full Nameneutrophil cytosolic factor 2
Alias Symbols67 kDa neutrophil oxidase factor;chronic granulomatous disease, autosomal 2;NADPH oxidase activator 2;NCF-2;neutrophil cytosol factor 2;neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2);neutrophil NADPH oxidase factor 2;NOXA2;P67PHOX;P67-PHOX.
NCBI Gene Id4688
Protein NameP19878
Description of TargetNeutrophil cytosol factor 2
Uniprot IDhttps://www.uniprot.org/uniprot/P19878
Protein Accession #NP_000424.2
Nucleotide Accession #NM_000433.3
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