NCAM1 Antibody : PE (OATA00337)

Catalog No: OATA00337

Human peripheral blood lymphocytes were stained with APC Anti-Human CD3 (OATA00014) and 5 uL (1 ug) PE Anti-Human CD56 (OATA00337) (right panel) or 1 ug PE Mouse IgG1 isotype control (left panel).

Human peripheral blood lymphocytes were stained with APC Anti-Human CD3 and the manufacturers recommended amount of PE Anti-Human CD56 (MY31) manufactured by Aviva Systems Biology (left panel) or BD Biosciences (right panel).

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Chimpanzee, Cynomolgus, Rhesus
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: MY31
• Isotype: Mouse IgG1, kappa
• Host: Mouse
• Conjugation: PE: Phycoerythrin
• Application: FC
  Excitation Laser: Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
  Emmision: 578 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD56
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Reference:

  Lanier LL, Testi R, Bindl J and Phillips JH. 1989. J Exp Med. 169: 2233-2238.

  Schlossman SF, Boumsell L, Gilks W et al., eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.

  Carter DL, Shieh TM, Blosser RL, Chadwick KR, Margolick JB, Hidreth JE, Clements JE and Zink MC. 1999. Cytometry. 37(1): 41-50.

  Chan WK, Suwannasaen D, Throm RE, Li Y, Eldridge PW, Houston J, Gray JT, Pui C-H and Leung W. 2015. Leukemia. 29: 387-395. (Flow cytometry)

  Woltman AM, Op den Brouw ML, Biesta PJ, Shi CC and Janssen HLA. 2011. PLoS ONE 6(1): e15324. doi: 10.1371/journal.pone.0015324. (Flow cytometry)

  Bleul CC, Wu L, Hoxie JA, Springer TA and Mackay CR. 1997. Proc Natl Acad Sci USA. 94(5): 1925-1930. (Flow cytometry)

  Brown K and Barratt-Boyes SM. 2009. J Med Primatol. 38(4): 272-278. (Flow cytometry - Rhesus)

Target Info

• Gene Symbol: NCAM1
• Gene Full Name: neural cell adhesion molecule 1
• Alias Symbols: CD56, NCAM, MSK39
• NCBI Gene Id: 4684
• Protein Name: neural cell adhesion molecule 1
• Description of Target: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.