NAXE ELISA Kit (Human) (OKCD00404)

Catalog No: OKCD00404 (Formerly GWB-KBBXF6)

Size:96WELLS

Human NAXE ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	85-96%	92-103%	95-103%	92-101%
  EDTA plasma(n=5)	83-105%	78-93%	82-91%	84-95%
  heparin plasma(n=5)	99-105%	95-105%	90-98%	85-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 Binding Protein (APOA1BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 Binding Protein (APOA1BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 Binding Protein (APOA1BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apolipoprotein A1 Binding Protein (APOA1BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	83-97	91
  EDTA plasma(n=5)	92-105	96
  heparin plasma(n=5)	86-94	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A1 Binding Protein (APOA1BP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A1 Binding Protein (APOA1BP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NAXE Microplate: 96 Wells (12 x 8 Well Strips)
  • NAXE Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated NAXE Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, urine and other biological fluids
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A1 Binding Protein (APOA1BP). No significant cross-reactivity or interference between Apolipoprotein A1 Binding Protein (APOA1BP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NAXE
• Gene Full Name: NAD(P)HX epimerase
• Alias Symbols: AIBP;AI-BP;APOA1BP;apoA-I binding protein;apolipoprotein A-I-binding protein;NAD(P)H-hydrate epimerase;NAD(P)HX epimerase;PEBEL;yjeF N-terminal domain-containing protein 1;yjeF_N1;YJEFN1.
• NCBI Gene Id: 128240
• Protein Name: NAD(P)H-hydrate epimerase
• Description of Target: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.
• Uniprot ID: Q8NCW5
• Protein Accession #: NP_658985
• Nucleotide Accession #: NM_144772.2
• Protein Size (# AA): 288