Nav1.8 Antibody (OASE00190)

Catalog No: OASE00190 (Formerly GWB-4628DF)

Size:100ug

Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone S64A-36. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-15. Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Nuclear staining. Magnification: 10X.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Monoclonal
• Clone: S134-12
• Isotype: IgG2a
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, AM
• Additional Information: Background Info: Detects ~220kDa. No cross reactivity against other Nav channels
  Scientific Background: Nav1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (3).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Nav1.8 in 10ug of COS cell lysate transiently expressing Nav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 1724-1956 of rat Nav1.8
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~220kDa. No cross reactivity against other Nav channels.
• Dilution: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA.
  2. www.iochannels.org
  3. Dray A. (2008) Br. J. Anaesth. 101(1): 48-58.
  4. Dray A., Read S.J (2007) Arthritis Res. Ther. 9(3): 212.
  5. Samuels M.E., teMorshe R.H., Lynch M.E., Drenth J.P. (2008) Mol Pain. 4: 21.
• Description: Nav1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals.

Target Info

• Gene Symbol: Nav1.8
• Gene Full Name: sodium channel, voltage-gated, type X, alpha subunit
• Alias Symbols: mPN3, Peripheral nerve sodium channel 3, Pn3 (gene name), PN3, Scn10a, Sensory neuron sodium channel, Sns (gene name), SNS, Sodium channel protein type 10 subunit alpha, Sodium channel protein type X alpha subunit, Voltage-gated sodium channel alpha subunit Nav1.8
• NCBI Gene Id: 29571
• Uniprot ID: Q62968
• Protein Accession #: NP_058943.1