Nav1.7 Antibody (OASE00164)

Catalog No: OASE00164 (Formerly GWB-86EB0F)

Size:100ug

Western Blot analysis of transiently transfected Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74. Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Nuclear staining of both neurons and glia.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Hamster
• Clonality: Monoclonal
• Clone: N68/6 (Formerly sold as S68-6)
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, IP, AM
• Additional Information: Background Info: Detects ~230kDa. No cross-reactivity against other Nav channels.
  Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Nav1.7 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.7 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.7 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (3-5).
  
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Nav1.7 in 10ug of HEK-293 cell lysate transiently expressing Nav1.7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 1751-1946 (C-terminus) of human Nav1.7
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~230kDa. No cross-reactivity against other Nav channels.
• Dilution: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA.
  2. www.iochannels.org
  3. Dray A. (2008) Br. J. Anaesth. 101(1): 48-58.
  4. Dray A., Read S.J (2007) Arthritis Res. Ther. 9(3): 212.
  5. Samuels M.E., teMorshe R.H., Lynch M.E., Drenth J.P.
  (2008) Mol Pain. 4: 21.
• Description: Nav1.7 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.7 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.7 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (1-3).

Target Info

• Gene Symbol: Nav1.7
• Gene Full Name: sodium channel, voltage-gated, type IX, alpha subunit
• Alias Symbols: ETHA, hNE Na, NE NA, PN1, SCN9A, voltage gated sodium channel subunit alpha Nav1, peripheral sodium channel 1, neuroendocrine sodium channel
• NCBI Gene Id: 6335
• Uniprot ID: Q15858
• Protein Accession #: NP_002968.1