NARS Antibody - N-terminal region (OAAB07325)

Catalog No: OAAB07325

Size:400ul

Western blot analysis of NARS (arrow) using rabbit polyclonal NARS Antibody (N-term) (Cat. #OAAB07325). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NARS gene.

NARS is strongly supported by BioGPS gene expression data to be expressed in HEK293, HepG2

NARS Antibody (N-term) (Cat. #OAAB07325) western blot analysis in WiDr,HepG2 cell line lysates (35ug/lane).This demonstrates the NARS antibody detected the NARS protein (arrow).

NARS Antibody (N-term) (Cat. #OAAB07325)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NARS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

NARS Antibody (N-term) (Cat. #OAAB07325) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: FC, IHC-P, WB
• Additional Information: Cellular Location: Cytoplasm.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This NARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human NARS.
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Bovine, Monkey, Mouse
• Concentration: 2 mg/ml
• Sample Type Confirmation:

  NARS is strongly supported by BioGPS gene expression data to be expressed in HEK293, HepG2

• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50

Target Info

• Gene Symbol: NARS
• Alias Symbols: NARS, ASNRS, NEDMILG, NEDMILEG
• NCBI Gene Id: 4677
• Protein Name: Asparagine--tRNA ligase, cytoplasmic
• Description of Target: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.
• Uniprot ID: O43776
• Protein Accession #: NP_004530.1
• Molecular Weight: 63 kDa