Nap1l4 ELISA Kit (Rat) (OKCD01962)

Catalog No: OKCD01962 (Formerly GWB-KBBID1)

Size:96WELLS

Rat Nap1l4 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	88-97%	95-105%	79-96%	90-104%
  EDTA Plasma (n=5)	78-98%	81-104%	98-105%	92-101%
  Heparin Plasma (n=5)	81-101%	98-105%	86-101%	94-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleosome Assembly Protein 1 Like Protein 4 (NAP1L4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleosome Assembly Protein 1 Like Protein 4 (NAP1L4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleosome Assembly Protein 1 Like Protein 4 (NAP1L4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nucleosome Assembly Protein 1 Like Protein 4 (NAP1L4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	80-90	84
  EDTA Plasma (n=5)	94-105	98
  Heparin Plasma (n=5)	81-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleosome Assembly Protein 1 Like Protein 4 (NAP1L4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleosome Assembly Protein 1 Like Protein 4 (NAP1L4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Nap1l4 Microplate: 96 Wells (12 x 8 Well Strips)
  • Nap1l4 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Nap1l4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.064 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nucleosome Assembly Protein 1 Like Protein 4 (NAP1L4). No significant cross-reactivity or interference between Nucleosome Assembly Protein 1 Like Protein 4 (NAP1L4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NAP1L4
• Gene Full Name: Nucleosome assembly protein 1-like 4
• Alias Symbols: Nucleosome assembly protein 1-like 4
• NCBI Gene Id: 361684
• Protein Name: Nucleosome assembly protein 1-like 4
• Description of Target: nucleosome assembly protein 1-like 4
• Uniprot ID: Q5U2Z3
• Protein Accession #: NP_001012170.1
• Nucleotide Accession #: NM_001012170.1
• Protein Size (# AA): 386