Catalog No: OPPA00165
Size:5UG
Price: $75.00
SKU
OPPA00165
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/Manuals | Printable datasheet for OPPA00165 |
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Predicted Species Reactivity | Human |
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Product Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
Host | Escherichia Coli. |
Additional Information | Solubility: It is recommended to Reconstitution: Reconstitute the lyophilized NANOG in sterile PBS not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
:: | Product Introduction: NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53. Product Description: NANOG Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 305 amino acids and having a molecular mass of 34.6kDa. The NANOG is fused to an N-terminal TAT (transcriptional activator protein) tag and purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Lyophilized NANOG although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution NANOG should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles. |
Formulation | NANOG was lyophilized after extensive dialysis against PBS. |
Purification | Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. |
Purity | Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. |
Peptide Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gly-Arg-Lys-Lys. |
Gene Symbol | NANOG-TAT |
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Alias Symbols | NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog. |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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