NANOG ELISA Kit (Mouse) (OKCD02749)

Catalog No: OKCD02749

Size:96WELLS

Mouse NANOG ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NANOG Homeobox Protein (NANOG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NANOG Homeobox Protein (NANOG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NANOG Homeobox Protein (NANOG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NANOG Homeobox Protein (NANOG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NANOG Homeobox Protein (NANOG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NANOG Homeobox Protein (NANOG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NANOG Microplate: 96 Wells (12 x 8 Well strips)
  • NANOG Lyophilized Standard: 2
  • 100X Biotinylated NANOG Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.064 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of NANOG Homeobox Protein (NANOG). No significant cross-reactivity or interference between NANOG Homeobox Protein (NANOG) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Nanog
• Gene Full Name: Nanog homeobox
• Alias Symbols: 2410002E02Rik;early embryo specific expression NK family;early embryo specific expression NK-type homeobox protein;ecat;ecat4;EN;ENK;ES cell-associated protein 4;homeobox protein NANOG;homeobox transcription factor Nanog.
• NCBI Gene Id: 71950
• Protein Name: Homeobox protein NANOG
• Description of Target: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal (PubMed:25825768). Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (By similarity). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
• Uniprot ID: Q80Z64
• Protein Accession #: NP_001276757.1
• Nucleotide Accession #: NM_001289828.1
• Protein Size (# AA): 305