NANOG Antibody (OAPA00267)

Catalog No: OAPA00267 (Formerly GWB-BSP927)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Clonality: Monoclonal
• Clone: P5A10AT.
• Isotype: Mouse IgG2a heavy chain and kappa light chain.
• Host: Mouse
• Application: WB
  Product Type: Mouse Anti Human Monoclonal.
  Product Introduction: NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
• Reconstitution and Storage: For periods up to 1 month store at 4C, for longer periods of time, store at -20C. Prevent freeze thaw cycles.
  Stability: 12 months at -20C. 1 month at 4C.
• Immunogen: The immunogen for anti-NANOG antibody: anti-human NANOG mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NANOG amino acids 1-154 purified from E. coli.
• Purity: NANOG antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Application Info: NANOG antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500~1:1,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:50.

Target Info

• Gene Symbol: NANOG
• Alias Symbols: NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG Antibody, Homeobox Protein NANOG, Mouse Anti Human
• NCBI Gene Id: 748301
• Protein Name: Homeobox protein NANOG
• Description of Target: Mouse Anti Human Homeobox Protein NANOG
• Uniprot ID: Q3LTE0