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Catalog No: OOPA00138 (Formerly GWB-BSP251)
Size:5UG
Price: $75.00
SKU
OOPA00138
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NANA E.Coli Protein (OOPA00138)

Datasheets/ManualsPrintable datasheet for OOPA00138
Product Info
Predicted Species ReactivityEscherichia coli
Product FormatSterile Filtered clear solution.
HostEscherichia Coli.
::Product Introduction: N-acetylneuraminate lyase (NanA) is a member of a family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. NanA is inhibited by reduction with NaBH4 in the presence of the substrate, which indicates that it belongs to the Schiff-base-forming Class I aldolases. NanA is strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, it is also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde.
Product Description: NANA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 317 amino acids (1-297 a.a.) and having a molecular mass of 34.7kDa. NANA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Reconstitution and StorageNANA E.Coli Recombinant although stable at 4C for 1 week, should be stored below -18C. Please prevent freeze thaw cycles.
FormulationThe NANA protein solution (1mg/ml) 20mM Tris-HCl buffer (pH8.0) and 20% glycerol.
PurificationGreater than 95% as determined by SDS-PAGE.
PurityGreater than 95% as determined by SDS-PAGE.
Peptide SequenceMGSSHHHHHH SSGLVPRGSH MATNLRGVMA ALLTPFDQQQ ALDKASLRRL VQFNIQQGID GLYVGGSTGE AFVQSLSERE QVLEIVAEEA KGKIKLIAHV GCVSTAESQQ LAASAKRYGF DAVSAVTPFY YPFSFEEHCD HYRAIIDSAD GLPMVVYNIP ALSGVKLTLD QINTLVTLPG VGALKQTSGD LYQMEQIRRE HPDLVLYNGY DEIFASGLLA GADGGIGSTY NIMGWRYQGI VKALKEGDIQ TAQKLQTECN KVIDLLIKTG VFRGLKTVLH YMDVVSVPLC RKPFGPVDEK YLPELKALAQ QLMQERG.
Gene SymbolNANA
Alias SymbolsN-acetylneuraminate lyase, N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, NALase, Sialate lyase, Sialic acid aldolase, Sialic acid lyase, nanA, npl, b3225, JW3194.
Protein Size (# AA)Recombinant
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