Catalog No: OKCD07467
Size:96WELLS
Price: $750.00
SKU
OKCD07467
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Datasheets/ManualsPrintable datasheet for NAIP ELISA Kit (Human) (OKCD07467)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal Apoptosis Inhibitory Protein (NAIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuronal Apoptosis Inhibitory Protein (NAIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuronal Apoptosis Inhibitory Protein (NAIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuronal Apoptosis Inhibitory Protein (NAIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuronal Apoptosis Inhibitory Protein (NAIP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuronal Apoptosis Inhibitory Protein (NAIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
NAIP Microplate96 Wells (12 x 8 Well strips)
NAIP Lyophilized Standard2
100X NAIP HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.058ng/mL
Gene SymbolNAIP
Gene Full NameNLR family apoptosis inhibitory protein
Alias Symbolsbaculoviral IAP repeat-containing protein 1;BIRC1;neuronal apoptosis inhibitory protein;NLRB1;nucleotide-binding oligomerization domain, leucine rich repeat and BIR domain containing 1;psi neuronal apoptosis inhibitory protein;psiNAIP.
NCBI Gene Id4671
Protein NameBaculoviral IAP repeat-containing protein 1
Description of TargetAnti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
Uniprot IDQ13075
Protein Accession #NP_001333799.1
Nucleotide Accession #NM_001346870.1
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