Catalog No: OKCD08404
Size:96WELLS
Price: $800.00
SKU
OKCD08404
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Datasheets/ManualsPrintable datasheet for NAGLU ELISA Kit (Human) (OKCD08404)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acetyl Alpha-D-Glucosaminidase (NAGLU). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acetyl Alpha-D-Glucosaminidase (NAGLU). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acetyl Alpha-D-Glucosaminidase (NAGLU), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of N-Acetyl Alpha-D-Glucosaminidase (NAGLU) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Acetyl Alpha-D-Glucosaminidase (NAGLU) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Acetyl Alpha-D-Glucosaminidase (NAGLU) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
NAGLU Microplate96 Wells (12 x 8 Well strips)
NAGLU Lyophilized Standard2
100X NAGLU HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.113ng/mL
Gene SymbolNAGLU
Gene Full NameN-acetyl-alpha-glucosaminidase
Alias Symbolsalpha-N-acetylglucosaminidase;CMT2V;MPS3B;MPS-IIIB;N-acetyl-alpha-glucosaminidase;N-acetylglucosaminidase, alpha;NAG;testicular tissue protein Li 18;UFHSD.
NCBI Gene Id4669
Protein NameAlpha-N-acetylglucosaminidase
Description of TargetInvolved in the degradation of heparan sulfate.
Uniprot IDP54802
Protein Accession #NP_000254.2
Nucleotide Accession #NM_000263.3
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