NAAA ELISA Kit (Mouse) (OKEH07708)

Catalog No: OKEH07708

Size:96T

Mouse NAAA ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	98-108% (n = 20)	100-110% (n = 20)	82-93% (n = 20)	102-112% (n = 20)
  EDTA plasma(n=5)	82-94% (n = 20)	110-119% (n = 20)	104-116% (n = 20)	102-112% (n = 20)
  heparin plasma(n=5)	86-95% (n = 20)	105-115% (n = 20)	90-100% (n = 20)	99-109% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology NAAA ELISA Kit (OKEH07708) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-NAAA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NAAA and incubated. The NAAA found in the sample or standards competes with the biotinylated NAAA for limited binding sites on the immobilized anti-NAAA antibody. Excess unbound biotinylated NAAA and sample or standard NAAA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NAAA captured in the well and inversely proportional to the amount of NAAA which was contained in the sample or standard.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.9% (n = 20)
  Mean Inter-assay CV%: <=10.2% (n = 20)
• ELISA Kit Component:
  • Anti-NAAA Microplate: 96 Wells (12 x 8 Well strips)
  • NAAA Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X NAAA-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Degrades bioactive fatty acid amides to their corresponding acids, with a preference for N-palmitoylethanolamine.
  Subcellular Location: Lysosome
  Unigene: Mm.28890
  String: 10090.ENSMUSP00000108726
  KEGG: mmu:67111
  Pfam: PF02275
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.291ng/mL
• Specificity: Natural and recombinant Mouse N-acylethanolamine-hydrolyzing acid amidase
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Naaa
• Gene Full Name: N-acylethanolamine acid amidase
• Alias Symbols: 2210023K21Rik;3830414F09Rik;A;acylsphingosine deacylase NAAA;Asahl;ASAH-like protein;N-acylethanolamine-hydrolyzing acid amidase;N-acylsphingosine amidohydrolase-like.
• NCBI Gene Id: 67111
• Protein Name: Q9D7V9
• Description of Target: N-acylethanolamine-hydrolyzing acid amidase
• Uniprot ID: https://www.uniprot.org/uniprot/Q9D7V9
• Protein Accession #: NP_001157159.1
• Nucleotide Accession #: NM_001163687.1