NAAA ELISA Kit (Human) (OKEH07707)

Catalog No: OKEH07707

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology NAAA ELISA Kit (Human) (OKEH07707) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NAAA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NAAA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NAAA captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.2% (n = 20)
  Mean Inter-assay CV%: <=7.7% (n = 20)
• ELISA Kit Component:
  • NAAA Microplate: 96 Wells (12 x 8 Well strips)
  • NAAA Lyophilized Standard: 2
  • 100X Biotinylated NAAA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine > N-arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine.
  Subcellular Location: Lysosome
  Unigene: Hs.437365
  String: 9606.ENSP00000286733
  KEGG: hsa:27163
  MIM: 607469
  Pfam: PF02275
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 8.01pg/mL
• Specificity: Natural and recombinant Human N-acylethanolamine-hydrolyzing acid amidase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NAAA
• Gene Full Name: N-acylethanolamine acid amidase
• Alias Symbols: acid ceramidase-like protein;acylsphingosine deacylase NAAA;ASAHL;ASAH-like protein;N-acylethanolamine-hydrolyzing acid amidase;N-acylsphingosine amidohydrolase-like;PLT.
• NCBI Gene Id: 27163
• Protein Name: Q02083
• Description of Target: N-acylethanolamine-hydrolyzing acid amidase
• Uniprot ID: https://www.uniprot.org/uniprot/Q02083
• Protein Accession #: NP_001035861.1
• Nucleotide Accession #: NM_001042402.1