MYT1 Antibody (Phospho-Thr495) (OAAB16071)

Catalog No: OAAB16071

Size:400ul

The anti-Phospho-MYT1-T495 Antibody (Cat. # OAAB16071) is used in Western blot to detect Phospho-MYT1-T495 in HL60 (left) and SK-BR-3 (right) tissue lysates.

PKMYT1 is strongly supported by BioGPS gene expression data to be expressed in HL60

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Dot blot analysis of anti-Phospho-MYT1-T495 Antibody (Cat. # OAAB16071) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: DB, WB, IHC
• Additional Information: Precautions: Phospho-MYT1-T495 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development
  Cellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MYT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T495 of human MYT1.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  PKMYT1 is strongly supported by BioGPS gene expression data to be expressed in HL60

• Application Info: DB: 1:500
  WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: PKMYT1
• Alias Symbols: MYT1, PPP1R126
• NCBI Gene Id: 9088
• Protein Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q99640
• Protein Accession #: NP_004194.3
• Molecular Weight: 54521 Da