MYO5A Antibody (OAEB02186)

Catalog No: OAEB02186

Size:100UG

OAEB02186 (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IF
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: MYO5A
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: C-ETKQLELDLN
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3ug/ml.
• Other Applications Image 1 Data: OAEB02186 (1ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence. Data kindly provided by Dr. Rüdiger Rudolf, Karlsruhe, Germany
• Reference: * Guey LT, Garcia-Closas M, Murta-Nascimento C, Lloreta J, Palencia L, Kogevinas M, Rothman N, Vellalta G, Calle ML, Marenne G, Tardon A, Carrato A, Garcia-Closas R, Serra C, Silverman DT, Chanock S, Real FX, Malats N,; Genetic susceptibility to distinct bladder cancer subphenotypes.; European urology 2010 Feb 57 (2): 283-92.; Pubmed 19692168

Target Info

• Gene Symbol: Myo5a|MYO5A
• Gene Full Name: myosin VA
• Alias Symbols: 9630007J19Rik;AI413174;AI661011;d;d-120J;Db;Dbv;dilute lethal-20J protein;dilute myosin heavy chain, non-muscle;dilute-opisthotonus;Dop;f;flail;flailer;flr;GS1;M;MVa;My;MYH12;Myo;MYO5;myosin 5a;myosin V;myosin VA (heavy chain 12, myoxin);myosin, heavy polypeptide kinase;myosin-12;myosin-Va;MyoVA;myoxin;MYR12;Sev-1;unconventional myosin-Va.
• NCBI Gene Id: 17918|25017|4644
• Protein Name: Unconventional myosin-Va
• Uniprot ID: Q9Y4I1
• Protein Accession #: NP_000250.3
• Molecular Weight: 215; 212