MYLIP ELISA Kit (Human) (OKCA01214)

Catalog No: OKCA01214

Size:96 Wells

Human MYLIP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	105	100-110
  1:2	96	92-99
  1:4	89	84-94
  1:8	93	90-96

• ELISA Kit Principle: Aviva Systems Biology MYLIP ELISA Kit (Human) (OKCA01214) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYLIP has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYLIP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MYLIP captured in the well.
• ELISA Kit Range: 15.6 - 1,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	101	99-103
  EDTA Plasma (n=4)	90	85-95

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01214 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01214 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • MYLIP Microplate: 96 Wells (12 x 8 Well strips)
  • MYLIP Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated MYLIP Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 3.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: MYLIP
• Gene Full Name: myosin regulatory light chain interacting protein
• Alias Symbols: MIR, IDOL
• NCBI Gene Id: 29116
• Protein Name: E3 ubiquitin-protein ligase MYLIP
• Description of Target: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
• Uniprot ID: Q8WY64
• Protein Accession #: NP_037394.2
• Nucleotide Accession #: NM_013262.3