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Catalog No: OKCA01725
Size:96 Wells
Price: $754.00
SKU
OKCA01725
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat9288-98
1:28580-89
1:49692-100
1:89490-103
ELISA Kit PrincipleAviva Systems Biology Myl2 ELISA Kit (Mouse) (OKCA01725) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Myl2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Myl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Myl2 captured in the well.
ELISA Kit Range15.6 - 1,000 pg/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)8984-95
EDTA Plasma (n=4)10298-108
ELISA Kit Component
ComponentAmount
Myl2 Microplate96 Wells (12 x 8 Well strips)
Myl2 Lyophilized Standard2 x 1 ng
100X Biotinylated Myl2 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity3.9 pg/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolMYL2
Gene Full Namemyosin, light polypeptide 2, regulatory, cardiac, slow
Alias SymbolsMlc, Myl, MLC-, MLC-2, Mlc2v, Mylpc, MLC-2v, Gm32672, MLC-2s/v
NCBI Gene Id17906
Protein NameMyosin regulatory light chain 2, ventricular/cardiac muscle isoform
Description of TargetContractile protein that plays a role in heart development and function (PubMed:10409661). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (PubMed:22426213, PubMed:16908724, PubMed:10409661). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (PubMed:9422794).
Uniprot IDP51667
Protein Accession #NP_034991.3
Nucleotide Accession #NM_010861.3
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