MYH2 ELISA Kit (Mouse) (OKCD01180)

Catalog No: OKCD01180 (Formerly GWB-KBBYN7)

Size:96WELLS

Mouse Myh2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-92%	97-105%	96-105%	92-105%
  EDTA plasma(n=5)	89-96%	79-99%	85-93%	84-96%
  heparin plasma(n=5)	91-99%	94-103%	81-101%	78-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 2 (MYH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 2 (MYH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 2 (MYH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myosin Heavy Chain 2 (MYH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	101
  EDTA plasma(n=5)	97-105	101
  heparin plasma(n=5)	86-101	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 2 (MYH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 2 (MYH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Myh2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Myh2 Lyophilized Standard: 2 x 400 pg
  • 100X Biotinylated Myh2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 2.88 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2). No significant cross-reactivity or interference between Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MYH2
• Gene Full Name: myosin, heavy polypeptide 2, skeletal muscle, adult
• Alias Symbols: MHC2, MHC2A, MyHC-, Myh2a, Myhs-, Myhsf, Myhs-f, Myhsf1, Myhs-f1, MyHC-IIa
• NCBI Gene Id: 17882
• Protein Name: Myh2 protein
• Description of Target: Myh2 protein
• Uniprot ID: I6L963
• Protein Accession #: NP_001034634
• Nucleotide Accession #: NM_001039545.2
• Protein Size (# AA): 1598