Catalog No: OKEH03871
Size:96T
Price: $625.00
SKU
OKEH03871
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MYH2 ELISA Kit (Bovine) (OKEH03871) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine MYH2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MYH2 and incubated. The MYH2 found in the sample or standards competes with the biotinylated MYH2 for limited binding sites on the immobilized anti-bovine MYH2 antibody. Excess unbound biotinylated MYH2 and sample or standard MYH2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MYH2 captured in the well and inversely proportional to the amount of MYH2 which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Component
ComponentAmount
Anti-MYH2 Microplate96 Wells (12 x 8 Well strips)
MYH2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X MYH2-Biotin Complex1 x 60 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.049 ng/mL
SpecificityNatural and recombinant Bovine Myosin-2
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolMYH2
Gene Full Namemyosin, heavy chain 2
Alias SymbolsMyosin heavy chain 2;Myosin heavy chain 2a;Myosin heavy chain, skeletal muscle, adult 2.
NCBI Gene Id788772
Protein NameQ9BE41
Description of TargetMyosin-2
Uniprot IDhttps://www.uniprot.org/uniprot/Q9BE41
Protein Accession #NP_001159699.1
Nucleotide Accession #NM_001166227.1
Protein Size (# AA)1940
Molecular Weight223 kDa
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