- Gene Symbol:
- Official Gene Full Name:
- NCBI Gene Id:
- Alias Symbols:
- Description of Target:
- Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
- Protein Name:
- Predicted Species Reactivity:
- Sample Type:
- Serum, plasma and other biological fluids
- 0.994 ng/ml
- Kit Range:
- 1.875 ng/ml - 120 ng/ml
- Kit Duration:
- 90 Minutes
- Kit Principle:
- Aviva Systems Biology Myeloperoxidase ELISA Kit (Human) (OKIA00077) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Myeloperoxidase has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Myeloperoxidase - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Myeloperoxidase captured in the well.
- Kit Component:
|Anti-Human Myeloperoxidase Microplate||96 Wells (12 x 8 Well Strips)|
|20X Diluent Concentrate||50 mL|
|20X Wash Solution||50 mL|
|100X Enzyme-Antibody Conjugate||150 uL|
|Chromogen-Substrate Solution||12 mL|
|Stop Solution||12 mL|
|Human Myeloperoxidase Calibrator||1|
- Kit Detection Method:
- Colorimetric, OD450 nm
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express MPO.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express MPO.
- Predicted Homology Based on Immunogen Sequence:
- Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
- Reconstitution and Storage:
- Store as indicated in product manual.
- Product Format:
- In Solution
- Assay Info:
- Assay Methodology: Quantitative Sandwich ELISA