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Myeloperoxidase ELISA Kit (Human) (OKIA00077)

96 Wells
$419.00
In Stock
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Application:
ELISA
Gene Symbol:
MPO
Official Gene Full Name:
myeloperoxidase
NCBI Gene Id:
4353
Alias Symbols:
MPO
Description of Target:
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Protein Name:
Myeloperoxidase
Swissprot Id:
P05164
Predicted Species Reactivity:
Human
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
0.994 ng/ml
Kit Range:
1.875 ng/ml - 120 ng/ml
Kit Duration:
90 Minutes
Kit Principle:
Aviva Systems Biology Myeloperoxidase ELISA Kit (Human) (OKIA00077) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Myeloperoxidase has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Myeloperoxidase - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Myeloperoxidase captured in the well.
Kit Component:
ComponentAmount
Anti-Human Myeloperoxidase Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Myeloperoxidase Calibrator1
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MPO.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MPO.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Product Format:
In Solution
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Additional Information:
Intended Use: The Myeloperoxidase test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Myeloperoxidase in serum, plasma of Human.
:::
Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Myeloperoxidase antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN MYELOPEROXIDASE ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human MPO.
7. HUMAN MYELOPEROXIDASE CALIBRATOR
One vial containing a lyophilized Human Myeloperoxidase calibrator.

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