Catalog No: OKCD01290
Size:96WELLS
Price: $750.00
SKU
OKCD01290
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Myeloperoxidase Antibody ELISA Kit (Human) (OKCD01290) |
---|
Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||
ELISA Kit Duration | 2h, 30min | ||||||||||||||||||||
ELISA Kit Linearity |
| ||||||||||||||||||||
ELISA Kit Principle | The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Myeloperoxidase Antibody (Anti-MPO) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Myeloperoxidase Antibody (Anti-MPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||
ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||
ELISA Kit Recovery |
| ||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Myeloperoxidase Antibody (Anti-MPO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Myeloperoxidase Antibody (Anti-MPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||
Sensitivity | 1.12 ng/mL | ||||||||||||||||||||
Assay Info | Assay Method: Quantitative Reverse Capture Sandwich ELISA |
Gene Symbol | MPO |
---|---|
Gene Full Name | myeloperoxidase |
Alias Symbols | myeloperoxidase. |
NCBI Gene Id | 4353 |
Protein Name | Myeloperoxidase |
Description of Target | Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. |
Uniprot ID | P05164 |
Nucleotide Accession # | NM_000250.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review