Catalog No: OKCD01290
Size:96WELLS
Price: $750.00
SKU
OKCD01290
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Myeloperoxidase Antibody ELISA Kit (Human) (OKCD01290)

Datasheets/ManualsPrintable datasheet for Myeloperoxidase Antibody ELISA Kit (Human) (OKCD01290)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h, 30min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)78-101%78-93%98-105%83-94%
EDTA Plasma (n=5)79-93%90-101%79-98%96-105%
Heparin Plasma (n=5)79-90%96-103%98-105%87-94%
ELISA Kit PrincipleThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Myeloperoxidase Antibody (Anti-MPO) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Myeloperoxidase Antibody (Anti-MPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)87-10192
EDTA Plasma (n=5)95-10298
Heparin Plasma (n=5)81-9886
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Myeloperoxidase Antibody (Anti-MPO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Myeloperoxidase Antibody (Anti-MPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Myeloperoxidase antibody Microplate96 Wells (12 x 8 Well strips)
Myeloperoxidase antibody Lyophilized Standard2 x XXX
100X Detector Antibody1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.12 ng/mL
Assay InfoAssay Method: Quantitative Reverse Capture Sandwich ELISA
Gene SymbolMPO
Gene Full Namemyeloperoxidase
Alias Symbolsmyeloperoxidase.
NCBI Gene Id4353
Protein NameMyeloperoxidase
Description of TargetPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Uniprot IDP05164
Nucleotide Accession #NM_000250.1
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