MYDGF ELISA Kit (Mouse) (OKEI00375)

Catalog No: OKEI00375

Size:96 Wells

Mouse MYDGF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Mydgf ELISA Kit (Mouse) (OKEI00375) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mydgf has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mydgf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Mydgf
• ELISA Kit Range: 0.016 - 1 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Mydgf. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Mydgf using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • Mydgf Microplate: 96 Wells (12 x 8 Well strips)
  • Mydgf Lyophilized Standard: 2
  • 100X Mydgf HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 30X Wash Buffer: 1 x 30 mL
  • 100X Luminol Substrate: 1 x 10 mL
  • Substrate Diluent: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: 9.375 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MYDGF
• Alias Symbols: Il25, Ly6elg, D17Wsu104, D17Wsu104e
• NCBI Gene Id: 28106
• Protein Name: Myeloid-derived growth factor
• Description of Target: Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway.
• Uniprot ID: Q9CPT4
• Protein Accession #: NP_543027
• Nucleotide Accession #: NM_080837