MYD88 Antibody - middle region (OAAB05114)

Catalog No: OAAB05114

Size:200ul

Western blot analysis of MyD88 Antibody (Center) in HepG2 cell lysates (35ug/lane). MyD88 (arrow) was detected using the purified Pab.

MYD88 is strongly supported by BioGPS gene expression data to be expressed in HepG2

Western blot analysis of MyD88 Antibody (Center) in mouse lung tissue lysates (35ug/lane). MyD88 (arrow) was detected using the purified Pab.(2ug/ml)

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MyD88 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

MyD88 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IHC
• Additional Information: Function: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine.
  
  Cellular Location: Cytoplasm
  Tissue Location: Ubiquitous.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MyD88 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human MyD88.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  MYD88 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Application Info: FC: 0.059027777777778
  WB: 1:1000
  IHC-P: 1:10~50
• Other Applications Image 1 Data: MyD88 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: MYD88
• Alias Symbols: IMD68, MYD88D
• NCBI Gene Id: 4615
• Protein Name: Myeloid differentiation primary response protein MyD88
• Uniprot ID: Q99836
• Protein Accession #: NP_001166037.1
• Molecular Weight: 33233 Da