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MYCN ELISA Kit (Rat) (OKEH03709)

96 Wells
$519.00
In Stock
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Predicted Species Reactivity:
Rat
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology MYCN ELISA Kit (Rat) (OKEH03709) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mycn has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Mycn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mycn captured in well.
Kit Range:
0.312 - 20ng/ml
Kit Component:
ComponentAmount
Mycn Microplate96 Wells (12 x 8 Well strips)
Mycn Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Mycn Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
0.162 ng/mL
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Rat N-myc proto-oncogene protein
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol:
MYCN
Official Gene Full Name:
v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog
Alias Symbols:
Nmuc1, Nmyc, N-myc, Nmyc1, N-myc proto-oncogene protein
NCBI Gene Id:
298894
Protein Name:
N-myc proto-oncogene protein
Swissprot Id:
Q63379
Protein Accession #:
NP_001013114.1
Nucleotide Accession #:
NM_001013096.1
Protein Size (# AA):
462
Molecular Weight:
50 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Mycn.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Mycn.

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