MYBPC3 ELISA Kit (Chicken) (OKEH08829)

Catalog No: OKEH08829

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology MYBPC3 ELISA Kit (Chicken) (OKEH08829) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYBPC3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYBPC3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MYBPC3 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.8% (n = 20)
  Mean Inter-assay CV%: <=12.2% (n = 20)
• ELISA Kit Component:
  • MYBPC3 Microplate: 96 Wells (12 x 8 Well strips)
  • MYBPC3 Lyophilized Standard: 2
  • 100X Biotinylated MYBPC3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle A bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. May be involved in the early phase of myofibrillogenesis.
  Unigene: Gga.39829
  String: 9031.ENSGALP00000032013
  Pfam: PF00041
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken Myosin-binding protein C, cardiac-type
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MYBPC3
• Alias Symbols: C-protein, cardiac muscle isoform.
• Protein Name: Q90688
• Description of Target: Myosin-binding protein C, cardiac-type
• Uniprot ID: https://www.uniprot.org/uniprot/Q90688