MYBL2 Antibody - N-terminal region (OABB01074)

Catalog No: OABB01074

Size:100UG

Anti-B MyB antibody, OABB01074, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: MCF-7 Cell Lysate

Anti-B MyB antibody, OABB01074, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human MYBL2, 46.8 kDa (162aa tag+ M1-L245)
Lane 1: Recombinant Human MYBL2 Protein 10ng
Lane 2: Recombinant Human MYBL2 Protein 5ng
Lane 3: Recombinant Human MYBL2 Protein 2.5ng

Anti-B MyB antibody, OABB01074, IHC(P)
IHC(P): Human Tonsil Tissue

Anti-B MyB antibody, OABB01074, IHC(P)
IHC(P): Rat Brain Tissue

Anti-B MyB antibody, OABB01074, ICC
ICC: A549 Cell

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: WB: The detection limit for MYBL2 is approximately 0.5ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: MYBL2(V-MYB avian myeloblastosis viral oncogene homolog-like 2), also called MYB-RELATED GENE BMYB, is a protein that in humans is encoded by the MYBL2 gene. The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. Barletta et al.(1991) assigned the MYBL2 gene to chromosome Xq13. However, Noben-Trauth et al.(1996) demonstrated that this assignment was an error. Using mouse Mybl2 cDNA clones as probes, they assigned Mybl2 in an interspecific backcross panel to distal mouse chromosome 2. Using human cDNA probes in combination with fluorescence in situ hybridization analysis, they localized MYBL2 to chromosome 20q13.1, a region that is commonly deleted in myeloid disorders and shows high homology of synteny to mouse chromosome 2. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
  Immunocytochemistry : 0.5-1 ug/ml: Human: Mouse, Rat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Myb-related protein B(MYBL2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Target Info

• Gene Symbol: MYBL2
• Gene Full Name: MYB proto-oncogene like 2
• Alias Symbols: BMYB;B-MYB;myb-like protein 2;myb-related protein B;v-myb avian myeloblastosis viral oncogene homolog-like 2;v-myb myeloblastosis viral oncogene homolog-like 2.
• NCBI Gene Id: 4605
• Protein Name: Myb-related protein B
• Description of Target: Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
• Uniprot ID: P10244
• Molecular Weight: 78764 MW