MYB Antibody (OAEB02266)

Catalog No: OAEB02266 (Formerly GWB-CBECE1)

Size:100UG

OAEB02266 (xug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02266 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

EB07890 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with EDTA buffer pH 9, HRP-staining.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: MYB
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Zebrafish: 92%
• Peptide Sequence: C-QRHYNDEDPEKEKR
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Approx 75kDa band observed in Human Spleen lysates (calculated MW of 72.3kDa according to NP_005366.2). Recommended concentration: 0.3-1ug/ml.
  Immunohistochemistry: In paraffin embedded Humam Kidney shows cytoplasm staining of renal tubules and in Human Breast cytoplasm staining of ductal epithelial cells. Recommended concentration, 3-6ug/ml.
• Reference: * Clappier E, Cuccuini W, Kalota A, Crinquette A, Cayuela JM, Dik WA, Langerak AW, Montpellier B, Nadel B, Walrafen P, Delattre O, Aurias A, Leblanc T, Dombret H, Gewirtz AM, Baruchel A, Sigaux F, Soulier J.; The C-MYB locus is involved in chromosomal translocation and genomic duplications in human T-cell acute leukemia (T-ALL) - the translocation defining a new T-ALL subtype in very young children.; Blood. 2007 Apr 23; [Epub ahead of print]; Pubmed 17452517

Target Info

• Gene Symbol: Myb|MYB
• Gene Full Name: MYB proto-oncogene, transcription factor|myeloblastosis oncogene
• Alias Symbols: AI550390;c-myb;Cmyb;c-myb_CDS;efg;gag-myb protein;M16449;myb proto-oncogene protein;myeloblastosis proto-oncogene product;oncogene AMV;proto-oncogene c-Myb;transcriptional activator Myb;tumor-specific myb protein;v-myb avian myeloblastosis viral oncogene homolog.
• NCBI Gene Id: 17863|4602
• Protein Name: Transcriptional activator Myb
• Uniprot ID: P10242
• Protein Accession #: NP_005366.2
• Molecular Weight: 72.3