Catalog No: OKCD00610 (Formerly GWB-KBBDA2)
Size:96WELLS
Price: $750.00
SKU
OKCD00610
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Datasheets/ManualsPrintable datasheet for MX2 ELISA Kit (Human) (OKCD00610)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)88-102%85-96%96-103%94-103%
EDTA Plasma (n=5)80-98%95-103%80-103%79-102%
Heparin Plasma (n=5)79-94%80-102%88-95%79-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myxovirus Resistance 2 (MX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myxovirus Resistance 2 (MX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myxovirus Resistance 2 (MX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myxovirus Resistance 2 (MX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)97-104101
EDTA plasma(n=5)80-10391
heparin plasma(n=5)82-9289
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myxovirus Resistance 2 (MX2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myxovirus Resistance 2 (MX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MX2 Microplate96 Wells (12 x 8 Well Strips)
MX2 Lyophilized Standard2 x 10 ng
100X Biotinylated MX2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Myxovirus Resistance 2 (MX2). No significant cross-reactivity or interference between Myxovirus Resistance 2 (MX2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMX2
Gene Full NameMX dynamin like GTPase 2
Alias Symbolsinterferon-induced GTP-binding protein Mx2;interferon-regulated resistance GTP-binding protein MXB;MXB;myxovirus (influenza virus) resistance 2;myxovirus resistance protein 2;p78-related protein;second interferon-induced protein p78.
NCBI Gene Id4600
Protein NameInterferon-induced GTP-binding protein Mx2
Description of TargetInterferon-induced dynamin-like GTPase with potent antiviral activity against human immunodeficiency virus type 1 (HIV-1). Acts by targeting the viral capsid and affects the nuclear uptake and/or stability of the HIV-1 replication complex and the subsequent chromosomal integration of the proviral DNA. Exhibits antiviral activity also against simian immunodeficiency virus (SIV-mnd). May play a role in regulating nucleocytoplasmic transport and cell-cycle progression.
Uniprot IDP20592
Protein Accession #NP_002454
Nucleotide Accession #NM_002463.1
Protein Size (# AA)715
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