MX1 ELISA Kit (Human) (OKCD00718)

Catalog No: OKCD00718 (Formerly GWB-KBBWZ1)

Size:96WELLS

Human MX1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myxovirus Resistance 1 (MX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myxovirus Resistance 1 (MX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myxovirus Resistance 1 (MX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myxovirus Resistance 1 (MX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myxovirus Resistance 1 (MX1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myxovirus Resistance 1 (MX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MX1 Microplate: 96 Wells (12 x 8 Well Strips)
  • MX1 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated MX1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.64 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myxovirus Resistance 1 (MX1). No significant cross-reactivity or interference between Myxovirus Resistance 1 (MX1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MX1
• Gene Full Name: MX dynamin like GTPase 1
• Alias Symbols: IFI78;IFI-78K;interferon-induced GTP-binding protein Mx1;interferon-induced protein p78;interferon-inducible protein p78;interferon-regulated resistance GTP-binding protein MxA;lncMX1-215;MX;MxA;myxoma resistance protein 1;myxovirus (influenza virus) resistance 1, interferon-inducible protein p78;Myxovirus resistance protein 1.
• NCBI Gene Id: 4599
• Protein Name: Interferon-induced GTP-binding protein Mx1
• Description of Target: Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium channel activity of TRPCs.
• Uniprot ID: P20591
• Protein Accession #: NP_001138397
• Nucleotide Accession #: NM_001144925.2
• Protein Size (# AA): 662