MX1 ELISA Kit (Human) (OKCA00704)

Catalog No: OKCA00704

Size:96 Wells

Human MX1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	84	80-89
  1:2	94	91-99
  1:4	95	92-98
  1:8	106	98-110

• ELISA Kit Principle: Aviva Systems Biology MX1 ELISA Kit (Human) (OKCA00704) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MX1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MX1 captured in the well.
• ELISA Kit Range: 15.6 pg/mL-1,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95	90-99
  EDTA Plasma (n=4)	98	90-102

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level MX1 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level MX1 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • MX1 Microplate: 96 Wells (12 x 8 Well strips)
  • MX1 Lyophilized Standard: 2
  • 100X Biotinylated MX1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates, Cell Lysates
• Sensitivity: 3.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MX1
• Gene Full Name: MX dynamin like GTPase 1
• Alias Symbols: MX, MxA, IFI78, IFI-78K, lncMX1-215
• NCBI Gene Id: 4599
• Protein Name: Interferon-induced GTP-binding protein Mx1
• Description of Target: This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants.
• Uniprot ID: P20591
• Protein Accession #: NP_001138397.1
• Nucleotide Accession #: NM_001144925.2