MVP Antibody - C-terminal region (OAAB16556)

Catalog No: OAAB16556 (Formerly GWB-6WPH1H)

Size:400ul

The anti-MVP C-term Antibody (Cat.# OAAB16556) is used in Western blot to detect MVP in A375 lysate.

Western blot analysis of MVP (arrow) using rabbit polyclonal MVP C-term Antibody (Cat.#OAAB16556). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MVP gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human lung carcinoma with MVP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H292 cells using MVP Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC, ELISA
• Additional Information: Cellular Location: Cytoplasm. Nucleus, nuclear pore complex. Note=5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment
  Tissue Location: Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MVP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 863-893 amino acids from the C-terminal region of human MVP.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Intended Use: MVP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: MVP
• Alias Symbols: LRP, VAULT1
• NCBI Gene Id: 9961
• Protein Name: Major vault protein
• Description of Target: MVP, the major vault protein, is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance.
• Uniprot ID: Q14764
• Protein Accession #: NP_005106.2
• Molecular Weight: 99 kDa