MUP20 ELISA Kit (Mouse) (OKEH02418)

Catalog No: OKEH02418

Size:96T

Mouse MUP20 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MUP20 ELISA Kit (Mouse) (OKEH02418) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MUP20 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MUP20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MUP20 captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 103%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.3% (n = 20)
  Mean Inter-assay CV%: < 9.9% (n = 20)
• ELISA Kit Component:
  • Anti-MUP20 Microplate: 96 Wells (12 x 8 Well strips)
  • MUP20 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated MUP20 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.186 ng/mL
• Specificity: Natural and recombinant Mouse Major urinary protein 20
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Mup20
• Gene Full Name: major urinary protein 20
• Alias Symbols: da;darcin;Gm12560;major urinary protein 20;major urinary protein 24;Mup24.
• NCBI Gene Id: 381530
• Protein Name: Q5FW60
• Description of Target: Major urinary protein 20
• Uniprot ID: https://www.uniprot.org/uniprot/Q5FW60
• Protein Accession #: NP_001012323.1
• Nucleotide Accession #: NM_001012323.1
• Protein Size (# AA): 181
• Molecular Weight: 21 kDa