MUM1 ELISA Kit (Human) (OKEH04636)

Catalog No: OKEH04636

Size:96T

Human MUM1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology MUM1 ELISA Kit (Human) (OKEH04636) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MUM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for MUM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MUM1 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-MUM1 Microplate: 96 Wells (12 x 8 Well Strips)
  • MUM1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • MUM1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 32 pg/mL
• Specificity: Natural and recombinant Human PWWP domain-containing protein MUM1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PWWP3A
• Gene Full Name: PWWP domain containing 3A, DNA repair factor
• Alias Symbols: EXPAND1;HSPC211;melanoma associated antigen (mutated) 1;melanoma ubiquitous mutated protein;MUM1;MUM-1;mutated melanoma-associated antigen 1;protein expandere;PWWP domain-containing DNA repair factor 3A;PWWP domain-containing protein MUM1.
• NCBI Gene Id: 84939
• Protein Name: Q2TAK8
• Description of Target: PWWP domain-containing protein MUM1
• Uniprot ID: https://www.uniprot.org/uniprot/Q2TAK8
• Protein Accession #: NP_116242.2
• Nucleotide Accession #: NM_032853.3
• Protein Size (# AA): 710
• Molecular Weight: 79 KDa