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Catalog No: OKCD03810
Size:96WELLS
Price: $875.00
SKU
OKCD03810
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MUC5AC Chemi-Luminescent ELISA Kit (Rat) (OKCD03810)

Datasheets/ManualsPrintable datasheet for MUC5AC Chemi-Luminescent ELISA Kit (Rat) (OKCD03810)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype AC (MUC5AC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype AC (MUC5AC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Mucin 5 Subtype AC (MUC5AC) level in the sample or standard.
ELISA Kit Range54.9-40,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Muc5ac Microplate96 Wells (12 x 8 Well strips)
Muc5ac Lyophilized Standard2
100X Muc5ac Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.0549 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMuc5ac
NCBI Gene Id65188
Protein NameMucin
Description of TargetMucin
Uniprot IDQ62907
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