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Catalog No: OKEH00720
Size:96T
Price: $525.00
SKU
OKEH00720
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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MUC4 ELISA Kit (Human) : 96 Wells (OKEH00720)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MUC4 ELISA Kit (Human) (OKEH00720) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human MUC4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MUC4 and incubated. The MUC4 found in the sample or standards competes with the biotinylated MUC4 for limited binding sites on the immobilized anti-human MUC4 antibody. Excess unbound biotinylated MUC4 and sample or standard MUC4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MUC4 captured in the well and inversely proportional to the amount of MUC4 which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.9% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-MUC4 Microplate96 Wells (12 x 8 Well strips)
MUC4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X MUC4-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.08 ng/mL
SpecificityNatural and recombinant Human Mucin-4
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolMUC4
Gene Full Namemucin 4, cell surface associated
Alias Symbolsascites sialoglycoprotein;ASGP;HSA276359;MUC-4;mucin 4, tracheobronchial;mucin-4;pancreatic adenocarcinoma mucin;testis mucin;tracheobronchial mucin.
NCBI Gene Id4585
Protein NameQ99102
Description of TargetMucin-4
Uniprot IDhttps://www.uniprot.org/uniprot/Q99102
Protein Accession #NP_004523.3
Nucleotide Accession #NM_004532.5
Protein Size (# AA)2169
Molecular Weight231 kDa
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