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Catalog No: OKEH00546
Size:96T
Price: $600.00
SKU
OKEH00546
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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MUC2 ELISA Kit (Mouse) : 96 Wells (OKEH00546)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH00546
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MUC2 ELISA Kit (Mouse) (OKEH00546) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MUC2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MUC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MUC2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 7.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-MUC2 Microplate96 Wells (12 x 8 Well strips)
MUC2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated MUC2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.088 ng/mL
SpecificityNatural and recombinant Mouse Mucin-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMUC2
Gene Full Namemucin 2, oligomeric mucus/gel-forming
Alias SymbolsColonic mucin;Secreted gel-forming mucin.
NCBI Gene Id17831
Protein NameQ80Z19
Description of TargetMucin-2
Uniprot IDhttps://www.uniprot.org/uniprot/Q80Z19
Protein Accession #NP_002448.3
Nucleotide Accession #NM_002457.3
Protein Size (# AA)5179
Molecular Weight540 kDa
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