MUC1 ELISA Kit (Rat) (OKCD00728)

Catalog No: OKCD00728 (Formerly GWB-KBBGK3)

Size:96WELLS

Rat Muc1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	95-105%	79-101%	84-93%	82-89%
  EDTA plasma(n=5)	94-102%	93-101%	85-92%	93-101%
  heparin plasma(n=5)	97-105%	78-91%	86-96%	98-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 1 (MUC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 1 (MUC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 1 (MUC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mucin 1 (MUC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	95-102	98
  EDTA plasma(n=5)	93-105	101
  heparin plasma(n=5)	80-97	80

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 1 (MUC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 1 (MUC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Muc1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Muc1 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated Muc1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
• Sensitivity: < 0.126 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mucin 1 (MUC1). No significant cross-reactivity or interference between Mucin 1 (MUC1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Muc1
• Gene Full Name: mucin 1, cell surface associated
• Alias Symbols: mucin 1, transmembrane;mucin-1;Mucin1.
• NCBI Gene Id: 24571
• Protein Name: Muc1 protein
• Description of Target: Muc1 protein
• Uniprot ID: B2GV31
• Protein Accession #: NP_036734
• Nucleotide Accession #: NM_012602.1
• Protein Size (# AA): 655