MUC1 ELISA Kit (Human) (OKCD06323)

Catalog No: OKCD06323

Size:96WELLS

MUC1 ELISA Kit (Human) (OKCD06323) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 1 (MUC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 1 (MUC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 1 (MUC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mucin 1 (MUC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 1 (MUC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 1 (MUC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MUC1 Microplate: 96 Wells (12 x 8 Well strips)
  • MUC1 Lyophilized Standard: 2
  • 100X MUC1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.266ng/mL

Target Info

• Gene Symbol: MUC1
• Gene Full Name: mucin 1, cell surface associated
• Alias Symbols: ADMCKD;ADMCKD1;ADTKD2;breast carcinoma-associated antigen DF3;CA 15-3;cancer antigen 15-3;carcinoma-associated mucin;CD227;EMA;episialin;H23 antigen;H23AG;KL-6;krebs von den Lungen-6;MAM6;MCD;MCKD;MCKD1;MUC-1;MUC-1/SEC;MUC-1/X;MUC1/ZD;mucin 1, transmembrane;mucin-1;peanut-reactive urinary mucin;PEM;PEMT;polymorphic epithelial mucin;PUM;tumor associated epithelial mucin;tumor-associated epithelial membrane antigen;Tumor-associated mucin.
• NCBI Gene Id: 4582
• Protein Name: Mucin-1
• Description of Target: The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
• Uniprot ID: P15941
• Protein Accession #: NP_001018016.1
• Nucleotide Accession #: NM_001018016.2