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Catalog No: OKEH02417
Size:96T
Price: $625.00
SKU
OKEH02417
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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MTR ELISA Kit (Human) : 96 Wells (OKEH02417)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MTR ELISA Kit (Human) (OKEH02417) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human MTR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MTR and incubated. The MTR found in the sample or standards competes with the biotinylated MTR for limited binding sites on the immobilized anti-Human MTR antibody. Excess unbound biotinylated MTR and sample or standard MTR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MTR captured in the well and inversely proportional to the amount of MTR which was contained in the sample or standard.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-MTR Microplate96 Wells (12 x 8 Well strips)
MTR Lyophilized Standard2
Sample Diluent1 x 20 mL
100X PKD2-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.23 ng/mL
SpecificityNatural and recombinant Human Methionine synthase
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolMTR
Gene Full Name5-methyltetrahydrofolate-homocysteine methyltransferase
Alias Symbols5-methyltetrahydrofolate--homocysteine methyltransferase;5-methyltetrahydrofolate-homocysteine methyltransferase 1;cblG;cobalamin-dependent methionine synthase;HMAG;methionine synthase;MS;vitamin-B12 dependent methionine synthase.
NCBI Gene Id4548
Protein NameQ99707
Description of TargetMethionine synthase
Uniprot IDhttps://www.uniprot.org/uniprot/Q99707
Protein Accession #NP_000245.2
Nucleotide Accession #NM_000254.2
Protein Size (# AA)1265
Molecular Weight141 kDa
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