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MTOR ELISA Kit (Human) : 96 Wells (OKEH02402)

Catalog#: OKEH02402
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityHuman
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology MTOR ELISA Kit (Human) (OKEH02402) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MTOR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MTOR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MTOR captured in well.
Kit Range78 - 5000pg/mL
Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
Kit Component
MTOR Microplate96 Wells (12 x 8 Well strips)
MTOR Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated C9 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity39 pg/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Human Serine/threonine-protein kinase mTOR
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMTOR
Official Gene Full Namemechanistic target of rapamycin
Alias SymbolsFK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, mTOR, RAFT1, Rapamycin target protein 1, RAPT1, Ser
NCBI Gene Id2475
Protein NameSerine/threonine-protein kinase mTOR
Description of TargetThe protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
Swissprot IdP42345
Protein Accession #NP_004949.1
Nucleotide Accession #NM_004958.3
Protein Size (# AA)2549
Molecular Weight289 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express MTOR.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express MTOR.
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