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MTOR ELISA Kit (Human) : 96 Wells (OKEH02402)

96 Wells
$599.00
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Gene Symbol:
MTOR
Official Gene Full Name:
mechanistic target of rapamycin
NCBI Gene Id:
2475
Alias Symbols:
FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, mTOR, RAFT1, Rapamycin target protein 1, RAPT1, Ser
Description of Target:
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
Protein Name:
Serine/threonine-protein kinase mTOR
Swissprot Id:
P42345
Protein Accession #:
NP_004949.1
Nucleotide Accession #:
NM_004958.3
Predicted Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 39 pg/mL
Kit Range:
78 - 5000 pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology MTOR ELISA Kit (Human) (OKEH02402) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MTOR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MTOR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MTOR captured in well.
Kit Component:
ComponentAmount
MTOR Microplate96 Wells (12 x 8 Well strips)
MTOR Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated C9 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MTOR.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MTOR.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural human Serine/threonine-protein kinase mTOR. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
289 kDa
Protein Size (# AA):
2549

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