MTNR1B ELISA Kit (Human) (OKCD08500)

Catalog No: OKCD08500

Size:96WELLS

MTNR1B ELISA Kit (Human) (OKCD08500) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melatonin Receptor 1B (MTNR1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melatonin Receptor 1B (MTNR1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melatonin Receptor 1B (MTNR1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Melatonin Receptor 1B (MTNR1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melatonin Receptor 1B (MTNR1B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melatonin Receptor 1B (MTNR1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MTNR1B Microplate: 96 Wells (12 x 8 Well strips)
  • MTNR1B Lyophilized Standard: 2
  • 100X MTNR1B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.055ng/mL

Target Info

• Gene Symbol: MTNR1B
• Gene Full Name: melatonin receptor 1B
• Alias Symbols: FGQTL2;mel1b receptor;MEL-1B-R;melatonin receptor 1B variant b;melatonin receptor MEL1B;melatonin receptor type 1B;MT2.
• NCBI Gene Id: 4544
• Protein Name: Melatonin receptor type 1B
• Description of Target: High affinity receptor for melatonin. Likely to mediate the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.
• Uniprot ID: P49286
• Protein Accession #: NP_005950.1
• Nucleotide Accession #: NM_005959.3