Catalog No: OKCD09071
Size:96WELLS
Price: $825.00
SKU
OKCD09071
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Datasheets/ManualsPrintable datasheet for MTFMT ELISA Kit (Mouse) (OKCD09071)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitochondrial Methionyl tRNA Formyltransferase (MTFMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitochondrial Methionyl tRNA Formyltransferase (MTFMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitochondrial Methionyl tRNA Formyltransferase (MTFMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mitochondrial Methionyl tRNA Formyltransferase (MTFMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitochondrial Methionyl tRNA Formyltransferase (MTFMT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitochondrial Methionyl tRNA Formyltransferase (MTFMT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
MTFMT Microplate96 Wells (12 x 8 Well strips)
MTFMT Lyophilized Standard2
100X MTFMT HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.055ng/mL
Gene SymbolMtfmt
Gene Full Namemitochondrial methionyl-tRNA formyltransferase
Alias Symbols2310020P08Rik;methionyl-tRNA formyltransferase, mitochondrial.
NCBI Gene Id69606
Protein NameMethionyl-tRNA formyltransferase, mitochondrial
Description of TargetFormylates methionyl-tRNA in mitochondria. A single tRNA(Met) gene gives rise to both an initiator and an elongator species via an unknown mechanism (By similarity).
Uniprot IDQ9D799
Protein Accession #NP_081410.2
Nucleotide Accession #NM_027134.3
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