Catalog No: OKEH04649
Size:96T
Price: $525.00
SKU
OKEH04649
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology MTBP ELISA Kit (Human) (OKEH04649) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MTBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for MTBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MTBP captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-MTBP Microplate96 Wells (12 x 8 Well Strips)
MTBP Lyophilized Standard2
Sample Diluent1 x 20 mL
MTBP Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.21 ng/mL
SpecificityNatural and recombinant Human Mdm2-binding protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMTBP
Gene Full NameMDM2 binding protein
Alias SymbolsMDM2 (mouse double minute 2)-binding protein, 104kD;Mdm2, transformed 3T3 cell double minute 2, p53 binding protein binding protein, 104kDa;mdm2-binding protein;MDM2BP.
NCBI Gene Id27085
Protein NameQ96DY7
Description of TargetMdm2-binding protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q96DY7
Protein Accession #NP_071328.2
Nucleotide Accession #NM_022045.4
Protein Size (# AA)904
Molecular Weight102 KDa
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