MTAP Antibody (OAGA00741)

Catalog No: OAGA00741

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
OAGA00741 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
OAGA00741 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MTAP(OAGA00741) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse liver
  Cellular Localization: Cytoplasm
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 283 of MTAP (Uniprot ID#Q13126)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: MTAP
• Gene Full Name: S-methyl-5'-thioadenosine phosphorylase
• Alias Symbols: BDMF, MSAP, DMSFH, LGMBF, DMSMFH, c86fus, HEL-249
• NCBI Gene Id: 4507
• Protein Name: S-methyl-5'-thioadenosine phosphorylase
• Uniprot ID: Q13126
• Protein Accession #: NP_002442.2
• Molecular Weight: 31 kDa