MTA2 Antibody (OAPB00139)

Catalog No: OAPB00139 (Formerly GWB-29EA67)

Size:100UG

Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 μg /ml.

Immunofluorescence of PID in HeLa cells with PID antibody at 10 ug/mL.

Immunocytochemistry staining of HeLa using PID antibody at 10 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PID Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: PID antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: PID antibody was raised against a 17 amino acid peptide near the carboxy terminus of human PID. The immunogen is located within the last 50 amino acids of PID.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-MTA2 (OAPB00139) antibody is Catalog # OEPB02251
• Specificity: No cross response to MTA1.
• Application Info: PID antibody can be used for detection of PID by Western blot at 0.5 to 1 ug/ml. HeLa cell lysate can be used as positive control and a 75 kDa band can be detected. It is human, mouse, and rat reactive and has no cross response to MTA1. (Optimal dilution should be determined by user)
• Reference: Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7. ;Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9. ;Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 2000;288(5468):1053-8. ;Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T, Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.

Target Info

• Gene Symbol: MTA2
• Gene Full Name: metastasis associated 1 family member 2
• Alias Symbols: metastasis -associated gene 1-like 1;metastasis associated gene family, member 2;metastasis-associated 1-like 1;metastasis-associated protein 2;metastasis-associated protein MTA2;MTA1L1;MTA1-L1 protein;p53 target protein in deacetylase complex;PID.
• NCBI Gene Id: 9219
• Protein Name: Metastasis-associated protein MTA2|p53 target protein in deacetylase complex [Homo sapiens]
• Description of Target: May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.
• Uniprot ID: O94776
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/AAG02241
• Nucleotide Accession #: NM_004739.3
• Molecular Weight: Predicted: 73 kDa Observed: 75 kDa