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Catalog No: OKCD02736
Size:96WELLS
Price: $650.00
SKU
OKCD02736
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Datasheets/ManualsPrintable datasheet for MT2 ELISA Kit (Mouse) (OKCD02736)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)85-99%82-104%98-105%82-90%
EDTA Plasma (n=5)96-103%95-105%92-101%84-92%
Heparin Plasma (n=5)98-105%92-105%93-101%93-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metallothionein 2 (MT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Metallothionein 2 (MT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Metallothionein 2 (MT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Metallothionein 2 (MT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)92-10195
EDTA Plasma (n=5)90-9995
Heparin Plasma(n=5)94-10398
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Metallothionein 2 (MT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Metallothionein 2 (MT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MT2 Microplate96 Wells (12 x 8 Well strips)
MT2 Lyophilized Standard2
100X Biotinylated MT2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity14.4 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Metallothionein 2 (MT2). No significant cross-reactivity or interference between Metallothionein 2 (MT2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolMt2
Gene Full Namemetallothionein 2
Alias SymbolsAA409533;metallothionein-2;metallothionein-II;MT-;Mt-2;MT-II.
NCBI Gene Id17750
Protein NameMetallothionein-2
Description of TargetMetallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Uniprot IDP02798
Protein Accession #NP_032656.1
Nucleotide Accession #NM_008630.2
Protein Size (# AA)61
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